CD80, B7-1, Clone: MEM-233, Mouse Monoclonal antibody-Human, FITC; flow
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Catalog numberOBT4508F
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PricePlease ask
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Size0.1 mg
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Alternative name 1CD80, B7-1, Clone: MEM-233, Mab anti-Human, FITC; flow
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Alternative name 2CD80, B7-1, Clone: MEM-233, Mab antibody-Human, FITC; flow
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Alternative name 3CD80, B7-1, Clone: MEM-233, Mab antibody-Homo sapiens, FITC; flow
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Alternative name 4anti-CD80 B7-1 Clone: MEM-233 flow
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Alternative name 5CD80 B7-1 Clone: MEM-233 flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD80, HHLA2, RNY4P2, LRRC23, PCDHGB7, NCR3LG1, CD274, CD86, TLX1NB, CD276
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Short nameCD80, B7-1, Clone: MEM-233, Mouse Monoclonal antibody- , FITC;
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD80 molecule, B7-1, clonality: MEM-233, Mouse monoclonal (antibody to-)-H. sapiens, fluorecein; flow
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD80 molecule, B7 and B7-1 and B7.1 and BB1 and CD28LG and CD28LG1 and LAB7, CD80 and IDBG-51287 and ENSG00000121594 and 941, coreceptor activity, Cell surfaces, Cd80 and IDBG-160288 and ENSMUSG00000075122 and 12519, BT.27986 and IDBG-632750 and ENSBTAG00000018059 and 407131
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Gene info
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Identity
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Gene
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Long gene nameCD80 molecule
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Synonyms gene
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Synonyms gene name
- CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
- CD80 molecule
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- C2-set domain containing
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHERV-H LTR-associating 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- V-set domain containing
- B7 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNY4 pseudogene 2
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Synonyms gene name
- RNA, Y4 small cytoplasmic (associated with Ro protein) pseudogene 2
- RNA, Ro-associated Y4 pseudogene 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-25
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameleucine rich repeat containing 23
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotocadherin gamma subfamily B, 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Clustered protocadherins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenatural killer cell cytotoxicity receptor 3 ligand 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2012-04-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C1-set domain containing
- B7 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD274 molecule
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Synonyms gene
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Synonyms gene name
- programmed cell death 1 ligand 1
- CD274 antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD86 molecule
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Synonyms gene
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Synonyms gene name
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTLX1 neighbor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2009-08-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long non-coding RNAs with non-systematic symbols
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD276 molecule
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Synonyms gene name
- CD276 antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID