CD63 Exo-Flow capture kit (Magnetic streptavidin beads, CD63-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)
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Catalog number
EXOFLOW300A-1
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Price
Please ask
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Size
10 reactions
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Shipping Temperature
Blue Ice
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Product category
Exosomes
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Properties
If you buy Antibodies supplied by Systembio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This Systembio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Systembio FITC is highly sensitive to pH extremes.
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Conjugation
Biotinylated, Anti-FITC Antibody, Magnetic beads
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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French translation
anticorps
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Gene target
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Gene symbol
CD63-AS1, CD63
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Short name
CD63 Exo- capture kit (Magnetic streptavidin , CD63- capture antibody, Elution Buffers, Exo-FITC stain)
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Technique
Antibody, FITC, Flow, antibodies against human proteins, antibodies for, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Fluorescein
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Label
Magnetic
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Alternative name
CD63 molecule Exo-Flow capture reagent (Magnetic biotinilated-binding streptavidin particles, CD63 molecule-biotinilated capture (antibody to-), washing and Elution Buffers, Exo-fluorecein staining)
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Alternative technique
antibodies, fluorescine, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene name
CD63 antisense RNA 1
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Locus
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Discovery year
2021-01-20
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Entrez gene record
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Classification
Gene info
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Identity
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Gene
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Long gene name
CD63 molecule
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Synonyms gene
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Synonyms gene name
- CD63 antigen (melanoma 1 antigen)
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Synonyms
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GenBank acession
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Locus
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Discovery year
1988-08-07
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Entrez gene record
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Classification
- CD molecules
- Tetraspanins
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
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Tree numbers
- E02.095.437
- E05.200.500.363.441
- E05.242.363.441
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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