CD58, LFA-3, Clone: BRIC-5, Mouse Monoclonal antibody-Human; flow/WB/Inhibits aggregation

  • Catalog number
    YSRTMCA912T
  • Price
    Please ask
  • Size
    25 ug
  • Alternative name 1
    CD58, LFA-3, Clone: BRIC-5, Mab anti-Human; flow/WB/Inhibits aggregation
  • Alternative name 2
    CD58, LFA-3, Clone: BRIC-5, Mab antibody-Human; flow/WB/Inhibits aggregation
  • Alternative name 3
    CD58, LFA-3, Clone: BRIC-5, Mab antibody-Homo sapiens; flow/WB/Inhibits aggregation
  • Alternative name 4
    anti-CD58 LFA-3 Clone: BRIC-5 flow//Inhibits aggregation
  • Alternative name 5
    CD58 LFA-3 Clone: BRIC-5 flow//Inhibits aggregation
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    CD58, TRI-AAT5-3, HS3ST5, TRY-GTA5-5, TRK-TTT3-3, ITGAL, H1-5, TRP-TGG3-5, TRF-GAA1-5, TRG-GCC2-5
  • Short name
    CD58, LFA-3, Clone: BRIC-5, Mouse Monoclonal antibody- ; / /Inhibits aggregation
  • Technique
    Mouse monoclonal, aggregation, Antibody, Mouse, Flow, WB, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals, Western Blot on a membrane
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Species
    Human, Humans, Mouses
  • Alternative name
    CD58 molecule, LFA-3, clonality: BRIC-5, Mouse monoclonal (antibody to-)-H. sapiens; flow/Western Blot/Inhibits aggregation
  • Alternative technique
    antibodies, murine
  • Alternative to gene target
    CD58 molecule, ag3 and LFA-3 and LFA3, CD58 and IDBG-101394 and ENSG00000116815 and 965, protein binding, Cell surfaces, CD58 and IDBG-634482 and ENSBTAG00000040131 and 782186
Gene info
  • Identity
  • Gene
  • Long gene name
    CD58 molecule
  • Synonyms gene
  • Synonyms gene name
    • CD58 antigen, (lymphocyte function-associated antigen 3)
  • GenBank acession
  • Locus
  • Discovery year
    1989-04-06
  • Entrez gene record
    965
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • CD molecules
    • Ig-like cell adhesion molecule family
    • MicroRNA protein coding host genes
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Ile (anticodon AAT) 5-3
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA isoleucine 17 (anticodon AAU)
    • transfer RNA-Ile (AAT) 5-5
    • transfer RNA-Ile (AAT) 5-3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    heparan sulfate-glucosamine 3-sulfotransferase 5
  • Synonyms gene name
    • heparan sulfate (glucosamine) 3-O-sulfotransferase 5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2003-06-11
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Sulfotransferases, membrane bound
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Tyr (anticodon GTA) 5-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA tyrosine 3 (anticodon GUA)
    • transfer RNA-Tyr (GTA) 5-3
    • transfer RNA-Tyr (GTA) 5-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Lys (anticodon TTT) 3-3
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA lysine 22 (anticodon UUU)
    • transfer RNA-Lys (TTT) 3-5
    • transfer RNA-Lys (TTT) 3-3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Pro (anticodon TGG) 3-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA proline 16 (anticodon UGG)
    • transfer RNA-Pro (TGG) 3-4
    • transfer RNA-Pro (TGG) 3-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Phe (anticodon GAA) 1-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA phenylalanine 6 (anticodon GAA)
    • transfer RNA-Phe (GAA) 1-3
    • transfer RNA-Phe (GAA) 1-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Gly (anticodon GCC) 2-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA glycine 16 (anticodon GCC)
    • transfer RNA-Gly (GCC) 2-3
    • transfer RNA-Gly (GCC) 2-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
MeSH Data
  • Name
  • Concept
    Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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