CD58 Antibody / LFA3

• Catalog number
  R32395
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CD58 / LFA3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (FFPE): 0.5-1ug/ml
• Notes
  Optimal dilution of the CD58 antibody should be determined by the researcher.
• Intented use
  This CD58 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P19256
• Purity
  Antigen affinity
• Description
  CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.
• Immunogen
  Amino acids F29-R215 from the human protein were used as the immunogen for the CD58 antibody.
• Storage
  After reconstitution, the CD58 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic, cell surface
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CD58   LFA3  
• Gene symbol
  CD58
• Short name
  Anti-CD58 / LFA3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to CD58 / LFA3
• Alternative technique
  antibodies
• Alternative to gene target
  CD58 molecule, ag3 and LFA-3 and LFA3, CD58 and IDBG-101394 and ENSG00000116815 and 965, protein binding, Cell surfaces, CD58 and IDBG-634482 and ENSBTAG00000040131 and 782186

Gene info

• Identity
  HGNC:1688
• Gene
  CD58
• Long gene name
  CD58 molecule
• Synonyms gene
  • LFA3
• Synonyms gene name
  • CD58 antigen, (lymphocyte function-associated antigen 3)
• GenBank acession
  • BC005930
• Locus
  1p13.1
• Discovery year
  1989-04-06
• Entrez gene record
  965
• Pubmed identfication
  • 9510189
• RefSeq identity
  • NM_001779
• Classification
  • CD molecules
  • Ig-like cell adhesion molecule family
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000022749

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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