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Long name
Mouse Monoclonal Antibody against Human CD5, Clone: L17F12
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Antigen
CD5
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
L17F12
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Applications
FC, IP, WB, ICC
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Immunogen
Human acute lymphoblastic leukemia (ALL) T cells
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Format conjugation
FITC
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Specificity
The mouse monoclonal antibody L17F12 reacts with CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
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Research area
CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD5, Clone: L17F12 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch Pathol Lab Med. 2007 May;131(5):748-54., *Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am J Clin Pathol. 2001 Dec;116(6):893-7., *McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a_x000D_ glycoprotein for superior characteristics in NMR experiments. Protein Eng. 1998 Oct;11(10):847-53., *Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric _x000D_ Oncology Group study. Blood. 1990 Jan 1;75(1):166-73., *Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1791-5.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
