COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)
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Catalog number
MBS570370
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Price
Please ask
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Size
50 Tests
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Products_type
Antibody
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Products_short_name
[CD45 (FITC) and CD14 (PE)]
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Products_gene_name
[CD45]
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Products_gene_name_syn
[CD14]
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Clone
[VIT200; MEM18]
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Reactivity
Human
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Specificity
The CD45 mAb (clone VIT200) recognizes a pan CD45 epitope. The CD14 mAb (clone MEM18) recognizes surface CD14 on human monocytes and macrophages as well as neutrophils. The sensitivity of CD45/CD14 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
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Form
PBS pH 7.2, 1% BSA, 0.05% NaN3
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Storage_stability
Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
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Tested_application
Direct Immunofluorescence (IF), Flow Cytometry (FC/FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD14, PTPRCAP, PTPRC
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Short name
COMBI IC Reagent: Mouse anti CD45 (FITC) Mouse anti CD14 (PE)
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Technique
Mouse, anti, FITC, antibody to, Fluorescein, mouses
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Host
Mouse
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Isotype
IgG2a; IgG1
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Label
FITC
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Species
Mouse, Mouses
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Alternative name
COMBI IC Reagent: Mouse antibody to CD45 (fluorecein) and Mouse antibody to CD14 molecule (peroxidase)
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD14 molecule, CD14 and IDBG-49157 and ENSG00000170458 and 929, lipoteichoic acid binding, Extracellular, Cd14 and IDBG-138525 and ENSMUSG00000051439 and 12475, CD14 and IDBG-646064 and ENSBTAG00000015032 and 281048
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Gene info
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Identity
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Gene
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Long gene name
CD14 molecule
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Synonyms gene name
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Locus
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Discovery year
1988-05-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Scavenger receptors
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VEGA ID
Gene info
Gene info
Product images
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