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Category
Antibody
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Concentration
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
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Form
Purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
CD44v4
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens). Other species not tested. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
FACS, IF, IHC-P
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Recommended dilutions
Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
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Notes
Titering of the CD44v4 antibody may be required for optimal performance.
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Intented use
This CD44v4 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P16070
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Purity
Purified by protein A Affinity Chromatography
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Description
This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
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Immunogen
An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the CD44v4 antibody.
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Storage
Store the CD44v4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Localization
Cell surface
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
