CD44, Pgp-1 activation T-Cell Marker, Clone: T2-F4, Rat Mouse Monoclonal antibody-Mouse, FITC
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Catalog numberDEV102-12-4/02
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PricePlease ask
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Size200ug
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Alternative name 1CD44, Pgp-1 activation T-Cell Marker, Clone: T2-F4, Rat Mab anti-Mouse, FITC
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Alternative name 2CD44, Pgp-1 activation T-Cell Marker, Clone: T2-F4, Rat Mab antibody-Mouse, FITC
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Alternative name 3CD44, Pgp-1 activation T-Cell Marker, Clone: T2-F4, Rattus norvegicus Mab antibody-Mus musculus, FITC
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Alternative name 4anti-CD44 Pgp-1 activation T-Cell Marker Clone: T2-F4
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Alternative name 5CD44 Pgp-1 activation T-Cell Marker Clone: T2-F4
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolHBA2, B3GALT2, GALNT2, HBG1, B4GALT2, CD44-AS1, ACTRT2, SLC25A5, HBZP1, B3GNT2
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Short nameCD44, Pgp-1 activation T- , Clone: T2-F4, Mouse Monoclonal antibody-Mouse, FITC
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TechniqueMouse monoclonal, activation, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostRat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesMouse, Mouses, Rats
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Alternative nameCD44 molecule (Indian blood group), Pgp-1 activation T-cellular detection labelled, clonality: T2-F4, Rat Mouse monoclonal (antibody to-)-Mouse, fluorecein
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD44 molecule (Indian blood group), CDW44 and CSPG8 and ECMR-III and HCELL and HUTCH-I and IN and LHR and MC56 and MDU2 and MDU3 and MIC4 and Pgp1, CD44 and IDBG-39556 and ENSG00000026508 and 960, hyaluronic acid binding, Cell surfaces, Cd44 and IDBG-193505 and ENSMUSG00000005087 and 12505, CD44 and IDBG-636564 and ENSBTAG00000011578 and 281057
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene namehemoglobin subunit alpha 2
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Synonyms gene name
- hemoglobin, alpha 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hemoglobin subunits
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namebeta-1,3-galactosyltransferase 2
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
- UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 3-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolypeptide N-acetylgalactosaminyltransferase 2
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Synonyms gene name
- UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Polypeptide N-acetylgalactosaminyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehemoglobin subunit gamma 1
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Synonyms gene name
- hemoglobin, gamma A
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Hemoglobin subunits
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namebeta-1,4-galactosyltransferase 2
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Synonyms gene name
- UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Beta 4-glycosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD44 antisense RNA 1
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Synonyms
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Locus
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Discovery year2017-02-10
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Pubmed identfication
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene nameactin related protein T2
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-11-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Actin related proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesolute carrier family 25 member 5
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Synonyms gene
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Synonyms gene name
- solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-08-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Solute carriers
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namehemoglobin subunit zeta pseudogene 1
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Synonyms gene
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Synonyms gene name
- hemoglobin, zeta pseudogene
- hemoglobin, zeta pseudogene 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
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Synonyms gene
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Synonyms gene name
- UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-05-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Beta 3-glycosyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data