CD44, Clone: OX-50, Mouse Monoclonal antibody-Rat; flow: FITC
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Catalog numberYSRTMCA643FB
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PricePlease ask
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Size0.5 mg
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Alternative name 1CD44, Clone: OX-50, Mab anti-Rat; flow: FITC
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Alternative name 2CD44, Clone: OX-50, Mab antibody-Rat; flow: FITC
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Alternative name 3CD44, Clone: OX-50, Mab antibody-Rattus norvegicustus norvegicus; flow: FITC
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Alternative name 4anti-CD44 Clone: OX-50 flow:
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Alternative name 5CD44 Clone: OX-50 flow:
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolIGLV1-50, HCRT, IGHV3-50, DCTN2, CD44-AS1, GAP43, CD200, TNFSF4, RNU6-50P, CENPU
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Short nameCD44, Clone: OX-50, Mouse Monoclonal antibody- ; : FITC
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesRat, Mouses, Rats
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Alternative nameCD44 molecule (Indian blood group), clonality: OX-50, Mouse monoclonal (antibody to-)-Rat; flow: fluorecein
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Alternative techniqueantibodies, murine, fluorescine
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Alternative to gene targetCD44 molecule (Indian blood group), CDW44 and CSPG8 and ECMR-III and HCELL and HUTCH-I and IN and LHR and MC56 and MDU2 and MDU3 and MIC4 and Pgp1, CD44 and IDBG-39556 and ENSG00000026508 and 960, hyaluronic acid binding, Cell surfaces, Cd44 and IDBG-193505 and ENSMUSG00000005087 and 12505, CD44 and IDBG-636564 and ENSBTAG00000011578 and 281057
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable 1-50 (non-functional)
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Synonyms gene name
- immunoglobulin lambda variable 1-50
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehypocretin neuropeptide precursor
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Synonyms gene name
- hypocretin (orexin) neuropeptide precursor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-50 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-50
- immunoglobulin heavy variable 3-50 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedynactin subunit 2
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Synonyms gene name
- dynactin 2 (p50)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-10-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Dynactin subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD44 antisense RNA 1
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Synonyms
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Locus
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Discovery year2017-02-10
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Pubmed identfication
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene namegrowth associated protein 43
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Synonyms
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Synonyms name
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Locus
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Discovery year1990-07-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD200 molecule
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Synonyms gene
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Synonyms gene name
- antigen identified by monoclonal antibody MRC OX-2
- CD200 antigen
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTNF superfamily member 4
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Synonyms gene
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Synonyms gene name
- tax-transcriptionally activated glycoprotein 1, 34kD
- tumor necrosis factor (ligand) superfamily, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-15
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 50, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear pseudogene 1
- RNA, U6 small nuclear 50
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namecentromere protein U
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Synonyms gene
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Synonyms gene name
- MLF1 interacting protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-07-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data