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Long name
Mouse Monoclonal Antibody against Human CD43 / Leukosialin, Clone: MEM-59
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Antigen
CD43
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
MEM-59
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Applications
FC, IP, WB, IHC(P), FUNC
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Immunogen
Human T lymphocytes.
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Format conjugation
APC-Cy 7
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Specificity
The antibody MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes._x000D_ HLDA IV; WS Code NL 604_x000D_ HLDA V; WS Code AS S290
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Research area
CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD43 / Leukosialin, Clone: MEM-59 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11, *Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33., *Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61. _x000D_ , *Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36._x000D_ , *Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995 Apr;25(4):1051-5. _x000D_ , *Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha). 1988;34(4):255-65. _x000D_ , *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
