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Long name
Mouse Monoclonal Antibody against Human CD41a, Clone: HIP8
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Antigen
CD41a
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Species reactivity
Human (Homo sapiens), Non-Human (Homo sapiens) Primates
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
HIP8
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Applications
FC, IHC(F)
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Format conjugation
purified; unconjugated
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Specificity
The antibody HIP8 reacts with alpha (a) subunit of CD41 (heavy chain; 120 kDa). CD41 is mainly expressed on platelets and megakaryocytes.The antibody HIP8 blocks platelet aggregation and completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. HIP8 is useful in the morphological and physiological studies of platelets and megakaryocytes._x000D_ HLDA IV; WS Code P 38
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Research area
CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Mouse Monoclonal Antibody against Human CD41a, Clone: HIP8 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-A affinity chromatography
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References
*Wu XW., Chen Z., Bao CX., et al., 1989: Production and application of anti-human platelet GPIIb/IIIa monoclonal antibodies. National Medical J. of China. 69(8):427, *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., *Leukocyte Typing IV., Knapp W et al. (Eds.), Oxford University Press (1989)., *Chen Z., Bao ZX., Yu AX., et al., 1987: A group monoclonal antibodies against human platelets with different functions. Chinese Science Bulletin. 24:1902
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
