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Alternative name 1
CD40, B-Cell marker, Clone: 3/23, Rat Mab anti-Mouse, ALEXA 647 conj.
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Alternative name 2
CD40, B-Cell marker, Clone: 3/23, Rat Mab antibody-Mouse, ALEXA 647 conj.
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Alternative name 3
CD40, B-Cell marker, Clone: 3/23, Rattus norvegicus Mab antibody-Mus musculus, ALEXA 647 conj.
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Alternative name 4
anti-CD40 B-Cell marker Clone: 3/23 ALEXA 647
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Alternative name 5
CD40 B-Cell marker Clone: 3/23 ALEXA 647
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate. If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Alexa Fluor
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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French translation
anticorps
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Gene target
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Gene symbol
CD40
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Short name
CD40, B- , Clone: 3/23, Mouse Monoclonal antibody-Mouse, 647 conj.
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Component cell markers and DNA bp. markers are genetic markers er genes or DNA sequences with a known location on a chromosome that can be used to identify individuals or species. Protein markers in kDA. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
Rat, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Label
ALEXA
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Species
Mouse, Mouses, Rats
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Alternative name
CD40 molecule, tumor necrosis factor receptor superfamily member 5, B-cellular detection labelled, clonality: 3/23, Rat Mouse monoclonal (antibody to-)-Mouse, ALEXA 647 conj.
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Alternative technique
antibodies, murine
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Alternative to gene target
CD40 molecule, TNF receptor superfamily member 5, Bp50 and CDW40 and p50 and TNFRSF5, CD40 and IDBG-78904 and ENSG00000101017 and 958, ubiquitin protein ligase binding, Extracellular, Cd40 and IDBG-212533 and ENSMUSG00000017652 and 21939, BT.42498 and IDBG-643036 and ENSBTAG00000020736 and 286849
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Tissue
cell
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