CD154 antibody biotin

• Catalog number
  61R-CD154aHUBT
• Price
  Please ask
• Size
  100 µg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Immunology
• Type of Immunogen
  CD154 antibody (biotin) was raised in mouse using human sgp39 fusion protein ..
• Raised in
  Mouse
• Specificity
  Human
• Cross Reactivity
  Should be determined by the end user
• Isotypes
  IgG1
• Clone
  24-31
• Conjugate Tag
  Biotin
• Grade Purity
  Grater than 95% pure
• Method of Purification
  CD154 antibody (biotin) was purified by Protein A chromatography.
• Concentration
  1.0 mg/ml
• Form Buffer
  50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; FC; IHC; WB
• Usage Recommendations
  FC: 10 ug/ml
• Additional Information
  This antibody immunoprecipitates a CD154 (gp39) molecule of about 39 kd. It also blocks MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation.
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• French translation
  anticorps

• Gene target
  CD154  
• Gene symbol
  CD40LG
• Short name
  CD154 antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  Mouse monoclonal CD154 antibody (biotin)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:11935
• Gene
  CD40LG
• Long gene name
  CD40 ligand
• Synonyms gene
  • HIGM1
  • IMD3
  • TNFSF5
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
• Synonyms
  • CD40L
  • TRAP
  • gp39
  • hCD40L
  • CD154
• Synonyms name
  • CD40 antigen ligand
  • tumor necrosis factor (ligand) superfamily member 5
  • T-B cell-activating molecule
  • TNF-related activation protein
  • hyper-IgM syndrome
• GenBank acession
  • X67878
• Locus
  Xq26.3
• Discovery year
  1989-06-30
• Entrez gene record
  959
• Pubmed identfication
  • 1427881
  • 7678782
• RefSeq identity
  • NM_000074
• Classification
  • CD molecules
  • Tumor necrosis factor superfamily
• VEGA ID
  OTTHUMG00000022512
• Locus Specific Databases
  • CD40Lbase: Mutation registry for X-linked Hyper-IgM syndrome
  • Global Variome shared LOVD
  • LRG_141

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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