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Alternative name 1
CD35, C3b Receptor (CR1), allotypes A (190kD)/ B (220kD)/ C (160kD)/ D (250kD), Clone: E11, Mab anti-Human, Monkey (Rhesus), Baboon, FITC; flow
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Alternative name 2
CD35, C3b Receptor (CR1), allotypes A (190kD)/ B (220kD)/ C (160kD)/ D (250kD), Clone: E11, Mab antibody-Human, Monkey (Rhesus), Baboon, FITC; flow
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Alternative name 3
CD35, C3b Receptor (CR1), allotypes A (190kD)/ B (220kD)/ C (160kD)/ D (250kD), Clone: E11, Mab antibody-Homo sapiens, Monkey (Rhesus), Baboon, FITC; flow
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Alternative name 4
anti-CD35 C3b Receptor CR1 alloty s A 190kDB 220kDC 160kDD 250kD Clone: E11 Rhesus flow
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Alternative name 5
CD35 C3b Receptor CR1 alloty s A 190kDB 220kDC 160kDD 250kD Clone: E11 Rhesus flow
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Clonality
Monoclonal Antibody
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Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Most human antibodies and protein are very homologous to baboon genes. BLASTed sequences are used to find homological regions. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Additional description
Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Monkeys like rhesus's or the rhesus macaque (Macaca mulatta) are the most used apes in laboraty models.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CR1
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Short name
CD35, C3b Receptor (CR1), allotypes A (190kD)/ B (220kD)/ C (160kD)/ D (250kD), Clone: E11, Mouse Monoclonal antibody- , ( ), , FITC;
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Technique
Mouse monoclonal, Antibody, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Label
FITC
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Species
Baboon, Baboons, Humans, Monkeys, Mouses, Rhesuses
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Alternative name
CD35, C3b Receptor (complement component (3b/4b) receptor 1 (Knops blood group)), allotypes A (190kD)/ B (220kD)/ C (160kD)/ D (250kD), clonality: E11, Mouse monoclonal (antibody to-)-H. sapiens, Monkey (Rhesus), Baboon, fluorecein; flow
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
complement component (3b/4b) receptor 1 (Knops blood group), C3BR and C4BR and CD35 and KN, CR1 and IDBG-106358 and ENSG00000203710 and 1378, complement component C3b receptor activity, Cell surfaces
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