CD34 FITC, Single-Color Reagent

Host Source

Mouse
Clone Name

CS37
Product Category

Flow Cytometry
Species Reactivity

Human
Application

Flow Cytometry
Immunogen

CD34=Derived from the hybridization of mouse Sp2/O-Ag14 cells with spleen cells of BALB/c mice immunized with human cell line KG-1a
Purification Method

Protein A/G Chromatography
Shipping Conditions

Room Temperature
Storage conditions

CD34 FITC, Single-Color Reagent should be stored at 4-8ºC. DO NOT FREEZE
Datasheet link
Properties

Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody

Gene target

CD34 Single-Color Reagent
Gene symbol

CD34
Short name

CD34 FITC, Single-Color Reagent
Technique

FITC, Fluorescein
Isotype

IgG1
Label

FITC
Alternative name

CD34 molecule fluorecein, Single-Color Reagent
Alternative technique

fluorescine
Alternative to gene target

CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051

Name

Cytophotometry
Concept

Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data