NK cell antibody FITC
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Catalog number61R-1160
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PricePlease ask
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Size50 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Raised inHamster
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SpecificityMouse
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IsotypesIgG
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Clone14B11
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Conjugate TagFITC
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Concentration500 ug/ml
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Form BufferProvided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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StorageStore at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditionsBlue Ice
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Tested forFC
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Usage RecommendationsFC: ≤ 0.25 ug/test
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Assay InformationA test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target-
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Gene symbolNCR1, NCR2, NKTR
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Short nameNK antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameSyrian Hamster monoclonal NK cell antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene namenatural cytotoxicity triggering receptor 1
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Synonyms gene
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Synonyms gene name
- lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-04-09
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenatural cytotoxicity triggering receptor 2
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Synonyms gene
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Synonyms gene name
- lymphocyte antigen 95 (activating NK-receptor; NK-p44)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-04-09
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Entrez gene record
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Pubmed identfication
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Classification
- CD molecules
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenatural killer cell triggering receptor
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Synonyms gene name
- natural killer-tumor recognition sequence
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-03-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclophilin peptidylprolyl isomerases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
