CD279, PD-1, Hamster Mouse Monoclonal antibody-Human; Clone: J43, Biotin conj.
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Catalog numberACL8982B
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PricePlease ask
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Size0.1mg
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Alternative name 1CD279, PD-1, Hamster Mab anti-Human; Clone: J43, Biotin conj.
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Alternative name 2CD279, PD-1, Hamster Mab antibody-Human; Clone: J43, Biotin conj.
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Alternative name 3CD279, PD-1, Hamster Mab antibody-Homo sapiens; Clone: J43, Biotin conj.
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Alternative name 4anti-CD279 PD-1 Clone: J43
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Alternative name 5CD279 PD-1 Clone: J43
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationBiotinylated
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolPDCD1, SNCA, ENPP2, SPATA2, ENPP3, PDCD1LG2, CD274, IGHVII-1-1, MIR1-1, TRUND-NNN8-1
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Short nameCD279, PD-1, Mouse Monoclonal antibody- ; Clone: J43, conj.
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TechniqueMouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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HostHamster, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelBiotin
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SpeciesHuman, Hamsters, Humans, Mouses
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Alternative nameCD279, PD-1, Hamster Mouse monoclonal (antibody to-)-H. sapiens; clonality: J43, biotinilated conj.
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene nameprogrammed cell death 1
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Synonyms gene
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Synonyms gene name
- systemic lupus erythematosus susceptibility 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-05-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesynuclein alpha
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Synonyms gene
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Synonyms gene name
- Parkinson disease (autosomal dominant, Lewy body) 4
- synuclein, alpha (non A4 component of amyloid precursor)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-01-24
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameectonucleotide pyrophosphatase/phosphodiesterase 2
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Synonyms gene
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-08-10
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Entrez gene record
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Pubmed identfication
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Classification
- Ectonucleotide pyrophosphatase/phosphodiesterase family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namespermatogenesis associated 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-03-15
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Entrez gene record
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameectonucleotide pyrophosphatase/phosphodiesterase 3
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-08-10
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Entrez gene record
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Pubmed identfication
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Classification
- Ectonucleotide pyrophosphatase/phosphodiesterase family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprogrammed cell death 1 ligand 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- Immunoglobulin like domain containing
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD274 molecule
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Synonyms gene
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Synonyms gene name
- programmed cell death 1 ligand 1
- CD274 antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data