CD1b,c (Thymocytes) (FITC)
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Catalog number
GEN608730
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Price
Please ask
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Size
100 Tests
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Also known as
CD1b,c
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Other names
CD1b antigen; T-cell surface glycoprotein CD1b; T-cell surface glycoprotein CD1b; OTTHUMP00000018908; OTTHUMP00000035065; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; CD1b molecule; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
CD1B; N/A; R1; CD1; CD1A; MGC125990; MGC125991; N/A
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Gene name synonims
CD1B; N/A; R1; CD1; CD1A; MGC125990; MGC125991; N/A
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Other gene names
CD1B; N/A; R1; CD1; CD1A; MGC125990; MGC125991; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1,k
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Clone
5J70
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Reacts with human CD1b and CD1c.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Highly Purified by Ion Exchange chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Conjugated to fluorescein isothiocyanate (FITC).
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
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Tested applications
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Short name
CD1b c (Thymocytes) (FITC)
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Technique
FITC, Fluorescein
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Label
FITC
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Alternative name
Anti-CD1b,c (Thymocytes) (FITC)
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Alternative technique
fluorescine
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Alternative to gene target
CD1b molecule, CD1 and CD1A and R1, CD1B and IDBG-103744 and ENSG00000158485 and 910, protein binding, Plasma membranes
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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