-
Alternative name 1
CD19 (Clone:HD37), RPE + Kappa Light Chain, Rabbit F(ab')2 anti-, FITC; flow
-
Alternative name 2
CD19 (Clone:HD37), RPE + Kappa Light Chain, Rabbit F(ab')2 antibody-, FITC; flow
-
Alternative name 3
CD19 (Clone:HD37), RPE + Kappa Light Chain, Rabbit F(ab')2 antibody-, FITC; flow
-
Alternative name 4
anti-CD19 Clone:HD37 + Kappa Light Chain F ab' 2 flow
-
Alternative name 5
CD19 Clone:HD37 + Kappa Light Chain F ab' 2 flow
-
Clonality
Monoclonal Antibody
-
Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
-
Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
-
Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
-
Group
f(ab')2s
-
Conjugation
Anti-FITC Antibody
-
Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
-
About
Rabbits are used for polyclonal antibody production by accurate-monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. accurate-monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
RPE, CD19
-
Short name
CD19 (Clone:HD37), RPE + Kappa Light Chain, Rabbit F(ab')2 antibody-, FITC;
-
Technique
Antibody, F(ab')2, Rabbit, light, FITC, Flow, antibodies against human proteins, antibodies for, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. Fluorescein
-
Host
Rabbit, Rabbits
-
Isotype
F(ab')2 Fragment Affinity-Purified Secondary Antibodies
-
Label
rpe
-
Alternative name
CD19 molecule (clonality:HD37), RPE + Kappa Light epitope, production species: rabbit F(Antibody')2 (antibody to-)-, fluorecein; flow
-
Alternative technique
antibodies, secondairy, rabbit-anti, fluorescine
-
Alternative to gene target
CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
-
