CD130, gp130 receptor, Clone: B-R3, Mouse Monoclonal antibody-Human, FITC; flow
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Catalog numberOBT4271F
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PricePlease ask
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Sizevial
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Alternative name 1CD130, gp130 receptor, Clone: B-R3, Mab anti-Human, FITC; flow
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Alternative name 2CD130, gp130 receptor, Clone: B-R3, Mab antibody-Human, FITC; flow
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Alternative name 3CD130, gp130 receptor, Clone: B-R3, Mab antibody-Homo sapiens, FITC; flow
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Alternative name 4anti-CD130 gp130 receptor Clone: B-R3 flow
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Alternative name 5CD130 gp130 receptor Clone: B-R3 flow
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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Additional descriptionThe receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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French translationanticorps
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Gene target
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Gene symbolSNRPB, ORM2, IL6ST, ERAL1, OR1B1, SPIB, EXPH5, HLA-B, RELB, RPS6KA4
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Short nameCD130, gp130 receptor, Clone: B-R3, Mouse Monoclonal antibody- , FITC;
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, Flow, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameCD130, gp130 receptor, clonality: B-R3, Mouse monoclonal (antibody to-)-H. sapiens, fluorecein; flow
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Alternative techniqueantibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene namesmall nuclear ribonucleoprotein polypeptides B and B1
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Synonyms gene
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Synonyms
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Locus
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Discovery year1988-11-28
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Entrez gene record
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Pubmed identfication
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Classification
- Small nucleolar RNA protein coding host genes
- Sm spliceosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameorosomucoid 2
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-03-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Lipocalins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 6 cytokine family signal transducer
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Synonyms gene name
- interleukin 6 signal transducer (gp130, oncostatin M receptor)
- interleukin 6 signal transducer
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-08-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibronectin type III domain containing
- CD molecules
- Interleukin receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEra like 12S mitochondrial rRNA chaperone 1
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Synonyms gene name
- Era (E. coli G-protein homolog)-like 1
- Era G-protein-like 1 (E. coli)
- Era-like 12S mitochondrial rRNA chaperone 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-11-19
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily B member 1
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Synonyms gene name
- olfactory receptor, family 1, subfamily B, member 1 (gene/pseudogene)
- olfactory receptor family 1 subfamily B member 1 (gene/pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-09
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Entrez gene record
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RefSeq identity
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Classification
- Olfactory receptors, family 1
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSpi-B transcription factor
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Synonyms gene name
- Spi-B transcription factor (Spi-1/PU.1 related)
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Synonyms
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Locus
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Discovery year1994-12-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ETS transcription factor family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameexophilin 5
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Synonyms
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Synonyms name
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Locus
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Discovery year2005-10-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, B
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Synonyms gene
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Synonyms gene name
- ankylosing spondylitis
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameRELB proto-oncogene, NF-kB subunit
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Synonyms gene name
- v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-10-02
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Entrez gene record
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Pubmed identfication
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Classification
- IPT domain containing
- NF-kappa B complex subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameribosomal protein S6 kinase A4
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Synonyms gene name
- ribosomal protein S6 kinase, 90kD, polypeptide 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-03-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- AGC family kinases
- MicroRNA protein coding host genes
- MAPK activated protein kinases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
