Cathepsin D Activity Fluorometric Assay Kit

• Catalog number
  K143-100
• Price
  Please ask
• Size
  100 assays

• Summary
  • Detection method- Fluorescence (Ex/Em 328/460) • Species reactivity- Mammalian • Kit size- 100 assays • Applications- Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
• Sample Type
  Cell and tissue lysates
• Features Benefits
  • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Cathepsin-D assay is straightforward, and can be adapted to 96-well plate assays.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  6-12 months
• Background
  Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-D Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Gene
  CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

• Gene target
  Cathepsin   Activity   Fluorometric   Kit  
• Short name
  Cathepsin D Activity Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Cathepsin D Activity fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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