Cathepsin B Antibody

• Catalog number
  R31477
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Cathepsin B
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Cathepsin B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Cathepsin B antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  1508
• Purity
  Antigen affinity
• Description
  Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene. The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimers disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that Cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimers disease. Overexpression has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene.
• Immunogen
  Human partial recombinant protein (AA 80-333) was used as the immunogen for this Cathepsin B antibody.
• Storage
  After reconstitution, the Cathepsin B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Gene
  CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
• French translation
  anticorps

• Gene target
  Cathepsin  
• Gene symbol
  CTSB, CTSZ, GZMB
• Short name
  Anti-Cathepsin B
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Cathepsin B
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2527
• Gene
  CTSB
• Long gene name
  cathepsin B
• GenBank acession
  • M14221
• Locus
  8p23.1
• Discovery year
  2001-06-22
• Entrez gene record
  1508
• Pubmed identfication
  • 8112600
  • 3463996
• RefSeq identity
  • NM_147780
• Classification
  • Cathepsins
• VEGA ID
  OTTHUMG00000090799

Gene info

• Identity
  HGNC:2547
• Gene
  CTSZ
• Long gene name
  cathepsin Z
• Synonyms
  • CTSX
• Synonyms name
  • cathepsin X
  • carboxypeptidase LB
  • cathepsin IV
  • cathepsin B2
  • cathepsin Y
  • cathepsin Z1
  • cysteine-type carboxypeptidase
  • lysosomal carboxypeptidase B
• GenBank acession
  • AF032906
• Locus
  20q13.32
• Discovery year
  1998-08-27
• Entrez gene record
  1522
• Pubmed identfication
  • 9642240
• RefSeq identity
  • NM_001336
• Classification
  • Cathepsins
• VEGA ID
  OTTHUMG00000032858

Gene info

• Identity
  HGNC:4709
• Gene
  GZMB
• Long gene name
  granzyme B
• Synonyms gene
  • CTLA1
  • CSPB
• Synonyms gene name
  • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Synonyms
  • CCPI
  • CGL-1
  • CSP-B
  • CGL1
  • CTSGL1
  • HLP
  • SECT
• Synonyms name
  • fragmentin 2
  • cytotoxic serine protease B
  • cathepsin G-like 1
  • T-cell serine protease 1-3E
  • granzyme 2
  • cytotoxic T-lymphocyte-associated serine esterase 1
• GenBank acession
  • BC030195
• Locus
  14q12
• Discovery year
  1989-05-12
• Entrez gene record
  3002
• Pubmed identfication
  • 2323780
• RefSeq identity
  • NM_004131
• Classification
  • Granule associated serine proteases of immune defence
• VEGA ID
  OTTHUMG00000029369

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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