Caspase-9 Substrate LEHD-AFC

• Catalog number
  1075-200
• Price
  Please ask
• Size
  200 assays

• Alternative_name
  Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic
• Description
  Ready-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also available separately.
• Structure Available
  No
• Peptide sequence
  Ac-Leu-Glu-His-Asp-AFC
• Salt Form
  No
• Molecular Formula
  C₃₃H₃₈F₃N₇O₁₁
• Molecular Weight
  765.7
• Cell Permeable
  No
• Purity
  >98% by HPLC
• Handling
  Protect from light and moisture
• Tag Line
  A caspase-9 substrate
• Storage Condition
  -20°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• MDL Number
  MFCD01862610
• PubChem CID
  44134612
• PubChem CID Link
• SMILES
  CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C
• InChi
  InChI=1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48)
• InChi Key
  HULKIXRFKRCRHD-UHFFFAOYSA-N
• Data Sheet Link
• MSDS Available
  Yes
• Additional description
  Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

• Gene target
  Caspase-9   Substrate   LEHD-AFC  
• Short name
  Caspase-9 Substrate LEHD-AFC
• Alternative name
  caspase-9 Substrate LEHD-AFC

MeSH Data

• Name
  Models, Chemical
• Concept
  Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
• Tree numbers
  • E05.599.495

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