Caspase-9 Proform, human recombinant
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Catalog number
1089P-50
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Price
Please ask
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Size
50 ug
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Synonyms
Caspase-9 Proform
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Alternative_names
Caspase-9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6, LEHDase
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Description
An aspartic acid protease involved in apoptosis
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Recombinant
Yes
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Source
E. coli.
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Purity by SDS PAGE
≥90%
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Assay
SDS-PAGE
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Concentration
2ug/ul
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Appearance
Liquid
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Physical form description
2 µg/µl in 1 mM acidic acid
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Background Information
Caspase-9 is a member of the caspase family of cysteine proteases. Caspase-9 exists in cells as an inactive proenzyme. Proteolytic processing of this inactive proenzyme generates the large and small subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be the key signal-transducing events in apoptosis. Caspase-9 is considered to be an upstream enzyme that initiates caspase cascade leading to apoptosis. The recombinant Procaspase-9 is produced by expression of a human cDNA in E. coli and further purified by multiple chromatographic steps.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Caspase-9 Proform, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
caspase-9 Proform, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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