Caspase 9 Fluorometric Assay Kit

4x Caspase 9 Fluorometric Assay Kit found in Biovision

  • Kit's description
    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader.
  • Highlights
    • Detection method: fluorecence (400 nm excitation filter and 505 nm emission filter) • Species reactivity: Mammalian • Kit size: Convenient sizes (25, 100, 200, 400 assays) • Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  • Kit's benefits
    • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.
  • Category
    Apoptosis Assay Kit
  • Kit's other name
    none
  • Contents
    • Cell Lysis Buffer • 2X Reaction Buffer • LEHD-AFC (1 mM) • DTT (1 M)
  • Storage condition
    -20°C
  • Shipping condition
    gel pack
  • Maximum time can be stored
    6-12 months
  • Species reactivity
    see datasheet
  • Applications
    Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  • Samples tested
    Cell and tissue lysates
  • Gene target
  • Short name
    Caspase-9 Fluorometric Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    caspase-9 fluorescence test reagent
  • Alternative technique
    arrays
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, this GO :0000187 and activation of MAPK activity and biological process this GO :0001541 and ovarian follicle development and biological process this GO :0001669 and acrosomal vesicle and cellular component this GO :0002020 and protease binding and molecular function this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002327 and immature B cell differentiation and biological process this GO :0002371 and dendritic cell cytokine production and biological process this GO :0002551 and mast cell chemotaxis and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0005020 and stem cell factor receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007286 and spermatid development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008354 and germ cell migration and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009314 and response to radiation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009898 and cytoplasmic side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0019955 and cytokine binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032762 and mast cell cytokine production and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0038093 and Fc receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038109 and Kit signaling pathway and biological process this GO :0038162 and erythropoietin-mediated signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043303 and mast cell degranulation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043473 and pigmentation and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048066 and developmental pigmentation and biological process this GO :0048070 and regulation of developmental pigmentation and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048565 and digestive tract development and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050673 and epithelial cell proliferation and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060374 and mast cell differentiation and biological process this GO :0070662 and mast cell proliferation and biological process this GO :0097067 and cellular response to thyroid hormone stimulus and biological process this GO :0097324 and melanocyte migration and biological process this GO :0097326 and melanocyte adhesion and biological process, transferase activity, this GO :0002020: protease binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0005020: stem cell factor receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019955: cytokine binding and also this GO :0042803: protein homodimerization activity and also this GO :0046872: metal ion binding, this GO :0002020: protease binding, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0005020: stem cell factor receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019955: cytokine binding, this GO :0042803: protein homodimerization activity, this GO :0046872: metal ion binding, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

K118-400 | Caspase-9 Fluorometric Assay Kit400 assays 1049.68 USD

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K118-25 | Caspase-9 Fluorometric Assay Kit25 assays 229.58 USD

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K118-100 | Caspase-9 Fluorometric Assay Kit100 assays 477.58 USD

  • Catalog number
    K118-100
  • Supplier
  • Price
    477.58 USD
  • Size
    100 assays
  • Test
    Biovision supplies other types of Assays as 1.
  • Description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
MeSH Data
  • Name
  • Concept
    Scope note:Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
  • Tree numbers
    • E05.599.495

K118-200 | Caspase-9 Fluorometric Assay Kit200 assays 676.46 USD

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