Caspase-6, human recombinant

  • Catalog number
    1086-25
  • Price
    Please ask
  • Size
    25 units
  • Synonyms
    Caspase-6
  • Alternative_names
    Caspase-6, CASP-6, Apoptotic protease Mch-2
  • Description
    A cysteine-aspartic acid protease involved in apoptosis
  • Recombinant
    Yes
  • Source
    E. coli.
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    > 13000 units/mg
  • Unit Definition
    One unit of the recombinant caspase-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • Molecular Weight
    large (18 kDa) and small (11 kDa)
  • Storage Temp
    -70°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized powder
  • Reconstitution Instructions
    Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
  • Background Information
    Caspase-6 (also know as Mch2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and henomic maintenance. The recombinant active human caspase-6 was expressed in E. coli. The active caspase-6 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VEID-pNA (Cat. #1070-200) or Ac-VEID-AFC (1069-200).
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Additional description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    CASP6, CARD6
  • Short name
    Caspase-6, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    caspase-6, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
  • Identity
  • Gene
  • Long gene name
    caspase 6
  • Synonyms gene name
    • caspase 6, apoptosis-related cysteine protease
    • caspase 6, apoptosis-related cysteine peptidase
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-07-22
  • Entrez gene record
    839
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Caspases
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    caspase recruitment domain family member 6
  • Synonyms gene name
    • caspase recruitment domain family, member 6
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-08-13
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Caspase recruitment domain containing
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
  • Tree numbers
    • E05.599.495
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