Caspase-8 Fluorometric Assay Kit

  • Catalog number
    K112-200
  • Price
    Please ask
  • Size
    200 assays
  • Description
    Convenient, Highly-Sensitive Assay
  • Summary
    • Detection method- Fluorescence (400 nm excitation filter and 505 nm emission filter) • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  • Sample Type
    Cell and tissue lysates
  • Features Benefits
    • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The FLICE/Caspase-8 Fluorometric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by FLICE or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
  • Test
    Biovision supplies other types of Assays as 1.
  • Additional description
    Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
  • Gene target
  • Short name
    Caspase-8 Fluorometric Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    caspase-8 fluorescence test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
  • Tree numbers
    • E05.599.495
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