Carboxyl Fluorescent Particles, UltraBlue, Flow Cytometry Grade, 1E7mL, 3.5-3.9µm, 2mL

• Catalog number
  CFP-3571-2
• Price
  Please ask
• Size
  2 mL

• Description
  Carboxyl Fluorescent Particles, UltraBlue, Flow Cytometry Grade, 1E7mL, 3.5-3.9µm are a used in cell biology assays such as cell counting, cell separation, flow cytometry (FC), calibration of flow cytometer machines and so on. Guaranteed to meet the size specification and produced from high quality materials, these microparticles will be a handy tool in your lab work.
• Notes
  For research use only.
• Additional description
  FACS or Flow Cytometry is an SSC and FSC analysis by scattered light gating. Becton, BD or coulter facses analyze for the major part human CD marker antibodies and cellular markers by PE or FITC labelled antibodies. Facses or flow cytometers will analyze forward and side scatters by gating of human lymphocytes. Becton Dickinson uses anti human CD antigens, CD4, CD8 monoclonals. The clones of these antibodies have a known affinity to these membrane receptors. Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.

• Gene target
  Carboxyl   Particles   UltraBlue   Grade   1E7mL   5-3   9µm   2mL  
• Gene symbol
  TRI-AAT5-3, HS3ST5, TRY-GTA5-5, TRK-TTT3-3, H1-5, TRF-GAA1-5, TRP-TGG3-5, TRG-GCC2-5, TRQ-CTG1-3, TRK-TTT3-5
• Short name
  Carboxyl Particles, UltraBlue, Grade, 1E7mL, 3 5-3 9µm, 2mL
• Technique
  Flow Cytometry, Flow
• Label
  fluorescent
• Alternative name
  Carboxyl Fluorescent particles, UltraBlue, Flow Cytometry Grade, 1E7mL, 3.5-3.9µm, 2mL

Gene info

• Identity
  HGNC:34877
• Gene
  TRI-AAT5-3
• Long gene name
  tRNA-Ile (anticodon AAT) 5-3
• Synonyms gene
  • TRNAI17
  • TRI-AAT5-5
• Synonyms gene name
  • transfer RNA isoleucine 17 (anticodon AAU)
  • transfer RNA-Ile (AAT) 5-5
  • transfer RNA-Ile (AAT) 5-3
• Synonyms
  • tRNA-Ile-AAT-5-3
• GenBank acession
  • HG983851
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189310
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:19419
• Gene
  HS3ST5
• Long gene name
  heparan sulfate-glucosamine 3-sulfotransferase 5
• Synonyms gene name
  • heparan sulfate (glucosamine) 3-O-sulfotransferase 5
• Synonyms
  • 3-OST-5
• GenBank acession
  • AF503292
• Locus
  6q21-q22.1
• Discovery year
  2003-06-11
• Entrez gene record
  222537
• Pubmed identfication
  • 12138164
• RefSeq identity
  • NM_153612
• Classification
  • Sulfotransferases, membrane bound
• VEGA ID
  OTTHUMG00000015412

Gene info

• Identity
  HGNC:34558
• Gene
  TRY-GTA5-5
• Long gene name
  tRNA-Tyr (anticodon GTA) 5-5
• Synonyms gene
  • TRNAY3
  • TRY-GTA5-3
• Synonyms gene name
  • transfer RNA tyrosine 3 (anticodon GUA)
  • transfer RNA-Tyr (GTA) 5-3
  • transfer RNA-Tyr (GTA) 5-5
• Synonyms
  • tRNA-Tyr-GTA-5-5
• GenBank acession
  • HG984080
• Locus
  14q11.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189001
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34797
• Gene
  TRK-TTT3-3
• Long gene name
  tRNA-Lys (anticodon TTT) 3-3
• Synonyms gene
  • TRNAK22
  • TRK-TTT3-5
• Synonyms gene name
  • transfer RNA lysine 22 (anticodon UUU)
  • transfer RNA-Lys (TTT) 3-5
  • transfer RNA-Lys (TTT) 3-3
• Synonyms
  • tRNA-Lys-TTT-3-3
• GenBank acession
  • HG983935
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189234
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:4719
• Gene
  H1-5
• Long gene name
  H1.5 linker histone, cluster member
• Synonyms gene
  • H1F5
  • HIST1H1B
• Synonyms gene name
  • H1 histone family, member 5
  • histone 1, H1b
  • histone cluster 1, H1b
  • histone cluster 1 H1 family member b
• Synonyms
  • H1b
  • H1s-3
• GenBank acession
  • AF531304
• Locus
  6p22.1
• Discovery year
  1997-08-29
• Entrez gene record
  3009
• Pubmed identfication
  • 9031620
  • 9119399
  • 12408966
• RefSeq identity
  • NM_005322
• Classification
  • H1 histones
• VEGA ID
  OTTHUMG00000016139

Gene info

• Identity
  HGNC:34814
• Gene
  TRF-GAA1-5
• Long gene name
  tRNA-Phe (anticodon GAA) 1-5
• Synonyms gene
  • TRNAF6
  • TRF-GAA1-3
• Synonyms gene name
  • transfer RNA phenylalanine 6 (anticodon GAA)
  • transfer RNA-Phe (GAA) 1-3
  • transfer RNA-Phe (GAA) 1-5
• Synonyms
  • tRNA-Phe-GAA-1-5
• GenBank acession
  • HG983962
• Locus
  13q32.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189251
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34847
• Gene
  TRP-TGG3-5
• Long gene name
  tRNA-Pro (anticodon TGG) 3-5
• Synonyms gene
  • TRNAP16
  • TRP-TGG3-4
• Synonyms gene name
  • transfer RNA proline 16 (anticodon UGG)
  • transfer RNA-Pro (TGG) 3-4
  • transfer RNA-Pro (TGG) 3-5
• Synonyms
  • tRNA-Pro-TGG-3-5
• GenBank acession
  • HG984000
• Locus
  16p13.3
• Discovery year
  2008-08-29
• Entrez gene record
  100189281
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34725
• Gene
  TRG-GCC2-5
• Long gene name
  tRNA-Gly (anticodon GCC) 2-5
• Synonyms gene
  • TRNAG16
  • TRG-GCC2-3
• Synonyms gene name
  • transfer RNA glycine 16 (anticodon GCC)
  • transfer RNA-Gly (GCC) 2-3
  • transfer RNA-Gly (GCC) 2-5
• Synonyms
  • tRNA-Gly-GCC-2-5
• GenBank acession
  • HG983813
• Locus
  16q22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189163
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34683
• Gene
  TRQ-CTG1-3
• Long gene name
  tRNA-Gln (anticodon CTG) 1-3
• Synonyms gene
  • TRNAQ11
  • TRQ-CTG1-5
• Synonyms gene name
  • transfer RNA glutamine 11 (anticodon CUG)
  • transfer RNA-Gln (CTG) 1-5
  • transfer RNA-Gln (CTG) 1-3
• Synonyms
  • tRNA-Gln-CTG-1-3
• GenBank acession
  • HG983720
• Locus
  6p22.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189121
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:12314
• Gene
  TRK-TTT3-5
• Long gene name
  tRNA-Lys (anticodon TTT) 3-5
• Synonyms gene
  • TRK1
  • TRNAK1
  • TRK-TTT3-4
• Synonyms gene name
  • tRNA lysine 1
  • tRNA lysine 1 (anticodon UUU)
  • transfer RNA lysine 1 (anticodon UUU)
  • transfer RNA-Lys (TTT) 3-4
  • transfer RNA-Lys (TTT) 3-5
• Synonyms
  • tRNA-Lys-TTT-3-5
• GenBank acession
  • HG983934
• Locus
  17p13.1
• Discovery year
  1989-06-30
• Entrez gene record
  7206
• Pubmed identfication
  • 6296772
• Classification
  • Cytoplasmic transfer RNAs

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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