C5a (His-Tag), human recombinant

  • Catalog number
    4995A-20
  • Price
    Please ask
  • Size
    20 ug
  • Synonyms
    Recombinant human C5a (His-Tag)
  • Alternative_names
    C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1
  • Description
    A potent chemotactic factor for human peripheral blood neutrophils and monocytes
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED₅₀ = 1.36 nmolar.
  • Molecular Weight
    (74 amino acids)
  • Storage Temp
    -20°C
  • Shipping
    Gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized protein
  • Physical form description
    Lyophilized with no additives
  • Reconstitution Instructions
    Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.
  • Background Information
    Human complement C5a is a 74-amino acid glycopeptide generated during complement activation from the a-chain of complement C5. C5a is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. The 74 amino acid, human C5a protein expressed in E. coli with a 6 amino acid, N-terminal His·Tag sequence and 28 amino acid proprietary vector sequence necessary for expression (C5a without His-tag is alailable, BioVision Cat # 4995B). The recombinant protein is not further processed to remove the additional sequence. Recombinant C5a has an activity similar to that of serum derived C5a based on enzyme assay of myeloperoxidase release.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Conjugation
    histidine
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    C5a   His-Tag  
  • Short name
    C5a (His-Tag), recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
  • Label
    His
  • Species
    Human, Humans
  • Alternative name
    C5a (histidine-detection labelled), H. sapiens Rec.
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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