C-RAF (Phospho-Thr269) Antibody

Applications

IHC ELISA
Source

Rabbit
Reactivity

H, M, R
MW kDa

73 kDa
Target Modification

Phospho
Modification Sites

H:T269 M:T269 R:T269
Immunogen

The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Thr269.
Immunogen Range

231-280
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

C-RAF (Phospho-Thr269) Antibody detects endogenous levels of C-RAF only when phosphorylated at Thr269.
Gene ID

5894
Swiss Prot No

P04049
OMIM No

164760/611553/611554
Unigene No

Hs.159130
NCBI Gene Symbol

RAF1
Synonyms

C-RAF; C-Raf; CRAF; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
Storage Stability

Stable at -20°C for at least 1 year.
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality

Polyclonal
Concentration

1 mg/ml
Properties

If you buy Antibodies supplied by Assay Biotech they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Identity

HGNC:14013
Gene

SMC4
Long gene name

structural maintenance of chromosomes 4
Synonyms gene
- SMC4L1
Synonyms gene name
- SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
- SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
Synonyms
- hCAP-C
- CAP-C
GenBank acession
- AF092564
Locus

3q25.33
Discovery year

2001-02-22
Entrez gene record

10051
Pubmed identfication
- 9789013
- 10319587
Classification
- Structural maintenance of chromosomes proteins
- Condensin I subunits
- Condensin II subunits
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000159006

Identity

HGNC:6776
Gene

MAF
Long gene name

MAF bZIP transcription factor
Synonyms gene name
- v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
Synonyms
- c-MAF
Locus

16q23.2
Discovery year

1991-08-01
Entrez gene record

4094
Pubmed identfication
- 14998484
Classification
- Basic leucine zipper proteins
VEGA ID

OTTHUMG00000137621

Identity

HGNC:9982
Gene

RFX1
Long gene name

regulatory factor X1
Synonyms gene name
- regulatory factor X, 1 (influences HLA class II expression)
Synonyms
- EF-C
Synonyms name
Locus

19p13.12
Discovery year

1991-11-06
Entrez gene record

5989
Pubmed identfication
- 1505960
- 8289803
RefSeq identity
- NM_002918
Classification
- Regulatory factor X family
VEGA ID

OTTHUMG00000181979

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

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