c-Fos Antibody: Biotin

Alternative Name

c-Fos Antibody, Clone 5B10: Biotin
Other name

Mouse Anti-Human c-fos Monoclonal IgG1
Target

c-fos
Conjugate

Biotin
Category

Antibodies
Product Type

Monoclonal
Clone Number

5B10
Immunogen

Synthetic peptide from the N-terminal of Human c-Fos (aa. 280-380)
Immunogen Species

Human
Gene ID

2353
Swiss Prot

P01100
Applications

WB
Host Species

Mouse
Species Reactivity

Human
Storage Buffer

PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration

1 mg/ml
Specificity

Detects 62 kDa band. Observed molecular weight of band is higher than predicted 41 kDa band due to post translational modifications.
Storage Temperature

-20°C
Shipping Temperature

Blue Ice or 4°C
Properties

If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Gene target

c-Fos
Gene symbol

FOS, USF2, VEGFD
Short name

c-Fos Antibody:
Technique

Antibody, antibodies against human proteins, antibodies for
Isotype

IgG1
Label

Biotin
Alternative name

c-FBJ murine osteosarcoma viral oncogene homolog (antibody to-): biotinilated
Alternative technique

antibodies
Alternative to gene target

FBJ murine osteosarcoma viral oncogene homolog, AP-1 and C-FOS and p55, FOS and IDBG-12957 and ENSG00000170345 and 2353, R-SMAD binding, nuclei, Fos and IDBG-158538 and ENSMUSG00000021250 and 14281, FOS and IDBG-646869 and ENSBTAG00000004322 and 280795

Identity

HGNC:3796
Gene

FOS
Long gene name

Fos proto-oncogene, AP-1 transcription factor subunit
Synonyms gene name
- v-fos FBJ murine osteosarcoma viral oncogene homolog
- FBJ murine osteosarcoma viral oncogene homolog
Synonyms
- c-fos
- AP-1
GenBank acession
- K00650
Locus

14q24.3
Discovery year

2001-06-22
Entrez gene record

2353
Pubmed identfication
RefSeq identity
- NM_005252
Classification
- Fos transcription factor family
- Basic leucine zipper proteins
VEGA ID

OTTHUMG00000171774

Identity

HGNC:3708
Gene

VEGFD
Long gene name

vascular endothelial growth factor D
Synonyms gene
- FIGF
Synonyms gene name
- c-fos induced growth factor (vascular endothelial growth factor D)
Synonyms
- VEGF-D
GenBank acession
- AJ000185
Locus

Xp22.2
Discovery year

1997-03-27
Entrez gene record

2277
Pubmed identfication
- 9479493
RefSeq identity
- NM_004469
Classification
- VEGF family
VEGA ID

OTTHUMG00000021175
Locus Specific Databases
- Global Variome shared LOVD

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data