Goat, DyLight® 488 conjugated

• Catalog number
  AS09 633
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Lyophilized
• Immunogen
  purified Rabbit IgG, whole molecule
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified goat IgG
• How to reconstitute
  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Verified applications
  immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF)
• Connected products
  other anti-rabbit DyLight® conjugated secondary antibodies Plant and algal protein extraction buffer
• Recommended dilutions for use
  1:20 - 1:2 000 (IHC), 1:50 - 1:5 000 (ICC), 1: 3000 (IF)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  rabbit IgG heavy and light chains (H&L)
• Possible reactivity
  rabbit IgG heavy and light chains (H&L)
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
• References
  Kaulmann et al. (2016). Inflammation related responses of intestinal cells to plum and cabbage digesta with differential carotenoid and polyphenol profiles following simulated gastro-intestinal digestion. Mol Nutr Food Res. 2016 Mar 18. doi: 10.1002/mnfr.201500947. Jimenez-Lopez et al. (2015). Biogenesis of protein bodies during legumin accumulation in developing olive (Olea europaea L.) seed. Protoplasma. 2015 May 21.
• Scientific context
  Goat anti-rabbit IgG, DyLight® 488 Conjugate is a secondary antibody conjugated to DyLight® 488, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
• Notes
  Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 488 has a maximum absorbance at 493 nm; Emax = 518 nm.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Capra aegagrus hircus

• Gene target
  DyLight®   488   conjugated  
• Short name
  Goat, DyLight® 488 conjugated
• Technique
  Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  DyLight
• Species
  Goats
• Alternative name
  caprine, DyLight® 488 coupled
• Alternative technique
  caprine

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