Bromelain, Bromelain, Affinity purified
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Catalog number
AS09 554
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Price
Please ask
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Size
1 mg
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Available ordering format
Lyophilized in phosphate buffer saline
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Immunogen
native bromelain isolated and purified from Ananas comosus
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgG
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How to reconstitute
For reconstitution please add 0.5 ml of sterile destilled water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
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Connected products
AS09 552 | anti-bromelainAS09 553 | anti-bromelain (biotinylated)AS09 546 | anti-PPI | papain (biotinylated)AS09 547 | anti-PPI | papain (affinity purified)Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 - 1: 100 000 for techniques listed above
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Ananas comosus
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Possible reactivity
Ananas comosus
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
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References
to be added when available
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Scientific context
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
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Notes
antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify Bromelain, Bromelain, by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
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Gene target
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Short name
Bromelain, Bromelain,
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Technique
affinity, purified, purifications
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Alternative name
Bromelain, Bromelain, protein enriched
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Alternative technique
pure
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