BRK, Active

• Catalog number
  7708-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  BRK, PTK6 protein tyrosine kinase 6
• Alternative_names
  BRK, PTK6 protein tyrosine kinase 6
• Description
  An intracellular signal transducer in epithelial tissues.
• Recombinant
  Yes
• Source
  Baculovirus (Sf9 insect cells)
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  133 nmol phosphate incorporated into Poly (Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 uCi/µl P-32.
• Molecular Weight
  ~80.0 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  5µg/50 µl
• Appearance
  Liquid
• Physical form description
  Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Background Information
  BRK is another member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domains as well as the catalytic domain (1). Although BRK shows strongest sequence similarity to members of the Src family, there are several key structural and regulatory differences that place it on its own amongst non-receptor PTKs. The genomic structure of BRK consists of 8 exons, whose boundaries are distinct from other non-receptor PTK family members (2). Alternate splicing of the primary BRK transcript generates a distinct mRNA which encodes a truncated protein consisting of an SH3 domain and a novel C-terminal proline rich sequence. Brk transcript is expressed in the human breast tumor cell line and expression of a tumor derived Brk cDNA in mouse embryonic fibroblasts and human mammary epithelial cells supports anchorage independent growth, and in the latter potentiates the mitogenic response to epidermal growth factor. Brk expression is low or undetectable in normal mammary tissue and benign lesions. However, approximately two-thirds of breast tumors express appreciable levels and 27% of tumors over express BRK by fivefold or more (up to 43x). This expression pattern is mirrored in comparison of cell lines derived either from normal mammary epithelial cells or from carcinomas (3).
• Datasheet

• Gene target
  BRK   Active  
• Gene symbol
  PTK6, BMPR2
• Short name
  BRK, Active
• Alternative name
  BRK, functionnal

Gene info

• Identity
  HGNC:9617
• Gene
  PTK6
• Long gene name
  protein tyrosine kinase 6
• Synonyms gene name
  • PTK6 protein tyrosine kinase 6
• Synonyms
  • BRK
• GenBank acession
  • U61412
• Locus
  20q13.33
• Discovery year
  1992-10-02
• Entrez gene record
  5753
• Pubmed identfication
  • 8247543
  • 9284935
• Classification
  • PTK6 family tyrosine kinases
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000033039

Gene info

• Identity
  HGNC:1078
• Gene
  BMPR2
• Long gene name
  bone morphogenetic protein receptor type 2
• Synonyms gene
  • PPH1
• Synonyms gene name
  • primary pulmonary hypertension 1
  • bone morphogenetic protein receptor, type II (serine/threonine kinase)
  • bone morphogenetic protein receptor type II
• Synonyms
  • BRK-3
  • T-ALK
  • BMPR3
  • BMPR-II
• GenBank acession
  • Z48923
• Locus
  2q33.1-q33.2
• Discovery year
  1997-03-19
• Entrez gene record
  659
• Pubmed identfication
  • 7791754
• RefSeq identity
  • NM_001204
• Classification
  • Type 2 receptor serine/threonine kinases
• VEGA ID
  OTTHUMG00000133617
• Locus Specific Databases
  • LRG_712

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