BRK, Active
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Catalog number7708-5
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Price:
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Size5 ug
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SynonymsBRK, PTK6 protein tyrosine kinase 6
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Alternative_namesBRK, PTK6 protein tyrosine kinase 6
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DescriptionAn intracellular signal transducer in epithelial tissues.
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RecombinantYes
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SourceBaculovirus (Sf9 insect cells)
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Activity Specifications test method133 nmol phosphate incorporated into Poly (Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 uCi/µl P-32.
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Molecular Weight~80.0 kDa
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Storage Temp-80°C
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ShippingDry ice
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Shelf Life12 months
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Concentration5µg/50 µl
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AppearanceLiquid
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Physical form descriptionRecombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
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Background InformationBRK is another member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domains as well as the catalytic domain (1). Although BRK shows strongest sequence similarity to members of the Src family, there are several key structural and regulatory differences that place it on its own amongst non-receptor PTKs. The genomic structure of BRK consists of 8 exons, whose boundaries are distinct from other non-receptor PTK family members (2). Alternate splicing of the primary BRK transcript generates a distinct mRNA which encodes a truncated protein consisting of an SH3 domain and a novel C-terminal proline rich sequence. Brk transcript is expressed in the human breast tumor cell line and expression of a tumor derived Brk cDNA in mouse embryonic fibroblasts and human mammary epithelial cells supports anchorage independent growth, and in the latter potentiates the mitogenic response to epidermal growth factor. Brk expression is low or undetectable in normal mammary tissue and benign lesions. However, approximately two-thirds of breast tumors express appreciable levels and 27% of tumors over express BRK by fivefold or more (up to 43x). This expression pattern is mirrored in comparison of cell lines derived either from normal mammary epithelial cells or from carcinomas (3).
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Gene target
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Gene symbolPTK6, BMPR2
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Short nameBRK, Active
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Alternative nameBRK, functionnal
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine kinase 6
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Synonyms gene name
- PTK6 protein tyrosine kinase 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-10-02
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Entrez gene record
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Pubmed identfication
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Classification
- PTK6 family tyrosine kinases
- SH2 domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebone morphogenetic protein receptor type 2
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Synonyms gene
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Synonyms gene name
- primary pulmonary hypertension 1
- bone morphogenetic protein receptor, type II (serine/threonine kinase)
- bone morphogenetic protein receptor type II
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-03-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Type 2 receptor serine/threonine kinases
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VEGA ID
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Locus Specific Databases