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Category
Other Reagents
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Long description
Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. _x000B_A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators (1-8). In these methods bromodeoxyuridine (BrdU), a thymidine analog replaces [3H] thymidine. BrdU is incorporated, into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA._x000B_Exalpha Biologicals BrdU Cell Proliferation Assay involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
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Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
BrdU Cell Proliferation Assay Kit
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Antibody is raised in
see techfile
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Antibody s reacts with
BrdU Incorporated Cells
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
ELISA
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Antibody s suited for
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation (2 µg/mg), Western blotting (1 µg/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 19: 131-138 (1996)._x000B__x000B_2. Wright WE, et al. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56:607._x000B__x000B_3. Aurade F, et al. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation 1994; 55:185.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Test
nordc supplies other types of Assays as 1.
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Description
For cells, cell lines and tissues in culture till half confluency.
