BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), Human recombinant

• Catalog number
  7651-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), Human recombinant
• Alternative_names
  Bromodomain containing 4; HUNK1; MCAP
• Description
  A protein involved in chromatin targeting
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Molecular Weight
  73.4 kDa (49-460 aa + GST Tag)
• Storage Temp
  -80°C
• Shipping
  dry ice
• Shelf Life
  6 months
• Appearance
  Liquid
• Physical form description
  50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol.
• Background Information
  The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  BRD4   bromodomains   49-460   GST-tagged  
• Gene symbol
  MGST3, IGLV9-49, IGHV3-49, IGHVII-49-1, NDUFS2, IFIT3, RNU6-49P, SCCPDH, BRD4, ZNF142
• Short name
  BRD4 bromodomains 1 2 (49-460 aa) (GST-tagged), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  BRD4 bromodomains 1 and 2 (49-460 aa) (GST-tagged), H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:5933
• Gene
  IGLV9-49
• Long gene name
  immunoglobulin lambda variable 9-49
• GenBank acession
  • Z73675
• Locus
  22q11.22
• Discovery year
  2000-05-08
• Entrez gene record
  28773
• RefSeq identity
  • NG_000002
• Classification
  • Immunoglobulin lambda locus at 22q11.2
• VEGA ID
  OTTHUMG00000151045

Gene info

• Identity
  HGNC:5607
• Gene
  IGHV3-49
• Long gene name
  immunoglobulin heavy variable 3-49
• GenBank acession
  • M99676
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28423
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151967

Gene info

• Identity
  HGNC:5683
• Gene
  IGHVII-49-1
• Long gene name
  immunoglobulin heavy variable (II)-49-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-49-1
  • immunoglobulin heavy variable (II)-49-1 pseudogene
• Synonyms
  • IGHV(II)-49-1
• GenBank acession
  • AB019438
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28364
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151974

Gene info

• Identity
  HGNC:7708
• Gene
  NDUFS2
• Long gene name
  NADH:ubiquinone oxidoreductase core subunit S2
• Synonyms gene name
  • NADH dehydrogenase (ubiquinone) Fe-S protein 2 (49kD) (NADH-coenzyme Q reductase)
  • NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)
• Synonyms
  • CI-49
• Synonyms name
  • complex I 49kDa subunit
  • NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
• GenBank acession
  • BC008868
• Locus
  1q23.3
• Discovery year
  1992-06-30
• Entrez gene record
  4720
• Pubmed identfication
  • 1832859
  • 9585441
• RefSeq identity
  • NM_004550
• Classification
  • NADH:ubiquinone oxidoreductase core subunits
• VEGA ID
  OTTHUMG00000034344

Gene info

• Identity
  HGNC:5411
• Gene
  IFIT3
• Long gene name
  interferon induced protein with tetratricopeptide repeats 3
• Synonyms gene
  • IFIT4
• Synonyms gene name
  • interferon-induced protein with tetratricopeptide repeats 4
  • interferon-induced protein with tetratricopeptide repeats 3
• Synonyms
  • ISG60
  • RIG-G
  • CIG-49
  • IFI60
  • GARG-49
  • IRG2
• GenBank acession
  • U52513
• Locus
  10q23.31
• Discovery year
  1998-08-13
• Entrez gene record
  3437
• Pubmed identfication
  • 9828129
  • 9391139
• RefSeq identity
  • NM_001549
• Classification
  • Tetratricopeptide repeat domain containing
• VEGA ID
  OTTHUMG00000018708

Gene info

• Identity
  HGNC:34293
• Gene
  RNU6-49P
• Long gene name
  RNA, U6 small nuclear 49, pseudogene
• Synonyms gene
  • RNU6-49
• Synonyms gene name
  • RNA, U6 small nuclear 49
• Locus
  Xp11.4
• Discovery year
  2008-06-10
• Entrez gene record
  106478923

Gene info

• Identity
  HGNC:24275
• Gene
  SCCPDH
• Long gene name
  saccharopine dehydrogenase (putative)
• Synonyms
  • CGI-49
  • NET11
• Locus
  1q44
• Discovery year
  2005-06-22
• Entrez gene record
  51097
• Pubmed identfication
  • 10810093
• RefSeq identity
  • NM_016002
• VEGA ID
  OTTHUMG00000040221

Gene info

• Identity
  HGNC:13575
• Gene
  BRD4
• Long gene name
  bromodomain containing 4
• Synonyms gene name
  • bromodomain-containing 4
• Synonyms
  • HUNKI
  • MCAP
  • CAP
  • HUNK1
• Synonyms name
  • chromosome-associated protein
  • mitotic chromosome-associated protein
• GenBank acession
  • Y12059
• Locus
  19p13.12
• Discovery year
  2000-09-26
• Entrez gene record
  23476
• Pubmed identfication
  • 10938129
• RefSeq identity
  • NM_058243
• Classification
  • Bromodomain containing
  • Bromodomain and extra-terminal domain family
• VEGA ID
  OTTHUMG00000183252

Gene info

• Identity
  HGNC:12927
• Gene
  ZNF142
• Long gene name
  zinc finger protein 142
• Synonyms gene name
  • zinc finger protein 142 (clone pHZ-49)
• Synonyms
  • KIAA0236
  • pHZ-49
• GenBank acession
  • U09849
• Locus
  2q35
• Discovery year
  1993-02-11
• Entrez gene record
  7701
• RefSeq identity
  • NM_005081
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000154736

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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