BRD3-Bromodomain2 (306-417 aa) (His-Tagged) human recombinant

• Catalog number
  7257-20
• Price
  Please ask
• Size
  20 ug

• Synonyms
  KIAA0043, RING3L
• Alternative_names
  KIAA0043, RING3L
• Description
  A protein involved in chromatin targeting
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Molecular Weight
  15.5 kDa
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  2 mg/ml
• Appearance
  Liquid
• Physical form description
  rh-BRD3-BD2 is supplied as a solution in PBS Buffer containing 10% glycerol.
• Background Information
  The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD3 binds hyper-acetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. It regulates transcription by promoting the binding of the transcription factor GATA1 to its targets and transcription of the CCND1 gene. A chromosomal aberration involving BRD3 was found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. The recombinant protein includes Bromodomain-containing protein 3 (contains 306-417 aa) with N-terminal His-tag.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  BRD3-Bromodomain2   306-417   His-Tagged  
• Gene symbol
  BRD3
• Short name
  BRD3-Bromodomain2 (306-417 aa) (His-Tagged) recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Label
  his-tagged
• Species
  Human, Humans
• Alternative name
  BRD3-Bromodomain2 (306-417 aa) (histidine-Tagged) H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1104
• Gene
  BRD3
• Long gene name
  bromodomain containing 3
• Synonyms gene name
  • bromodomain-containing 3
• Synonyms
  • RING3L
  • ORFX
  • KIAA0043
• Synonyms name
  • RING3-like
• Locus
  9q34.2
• Discovery year
  2000-07-25
• Entrez gene record
  8019
• Pubmed identfication
  • 7584044
  • 8781126
• RefSeq identity
  • NM_007371
• Classification
  • Bromodomain containing
  • Bromodomain and extra-terminal domain family
• VEGA ID
  OTTHUMG00000021004

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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