-
Target antigen
BRCA1
-
Clonality
Polyclonal antibody
-
Clone
Polyclonal antibody
-
Raised in
rabbit
-
Type of the antibody
IgG polyclonal antibody
-
Product form
freeze-dried
-
Reacts with species
human, mouse, rat
-
Analyses
WB,IHC-P
-
Immunogen
E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.
-
Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
-
Purification
Immunogen affinity purified.
-
Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
-
Storage condtions
Keep the BRCA1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
-
Tips
The BRCA1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
-
Background
BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.
-
Related articles
1. Lee, M.-H., Lahusen, T., Wang, R.-H., Xiao, C., Xu, X., Hwang, Y.-S., He, W.-W., Shi, Y., Deng, C.-X. Yin Yang 1 positively regulates BRCA1 and inhibits mammary cancer formation. Oncogene 31: 116-127, 2012. 2. Thompson, M. E., Jensen, R. A., Obermiller, P. S., Page, D. L., Holt, J. T. Decreased expression of BRCA1 accelerates growth and is often present during sporadic breast cancer progression. Nature Genet. 9: 444-450, 1995. 3. Wang, B., Hurov, K., Hofmann, K., Elledge, S. J. NBA1, a new player in the Brca1 A complex, is required for DNA damage resistance and checkpoint control. Genes Dev. 23: 729-739, 2009.
-
Gene Name
BRCA1
-
Protein Name
Breast cancer type 1 susceptibility protein
-
Gene Full Name
breast cancer 1, early onset
-
Synonyms
BRAC 1 antibody|BRCA 1 antibody|BRCA1 antibody|BRCA1/BRCA2 containing complex subunit 1 antibody|BRCA1/BRCA2-containing complex, subunit 1 antibody|BRCA1_HUMAN antibody|BRCAI antibody|BRCC 1 antibody|BRCC1 antibody|Breast and ovarian cancer susceptibility protein 1 antibody|Breast Cancer 1 antibody|Breast Cancer 1 Early Onset antibody|Breast cancer type 1 susceptibility protein antibody|Breast Ovarian Cancer Susceptibility antibody|Breast Ovarian Cancer Susceptibility antibody|BROVCA1 antibody|IRIS antibody|Papillary Serous Carcinoma Of The Peritoneum antibody|PNCA4 antibody|PPP1R53 antibody|Protein phosphatase 1 regulatory subunit 53 antibody|PSCP antibody|RING finger protein 53 antibody|RING finger protein 53 antibody|RNF53 antibody
-
Uniprot ID
P38398
-
Entrez GeneID
672
-
Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
