Bovine IgG Fc FITC
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Catalog number65C-CH0105
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PricePlease ask
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Size1 mg
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CategoryImmunoglobulins
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Antibody SubtypeConjugated Immunoglobulins
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Area of researchImmunology
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ReactivityBovine
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Conjugate TagFITC
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Method of PurificationBovine IgG (Fc) (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
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Form BufferLyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forIF
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Usage RecommendationsOptimal conditions to be determined by end user
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target-
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Gene symbolFCGBP, FCGR1A, FCRLB
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Short nameIgG Fc FITC
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TechniqueFITC, IgG, FC, Fluorescein, IgGs
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IsotypeIgG, IgG
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LabelFITC
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SpeciesBovine, Bos Taurus genes are available form the Bovine Genome database.
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Alternative namePurified Bovine IgG (Fc) FITC conjugate
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Alternative techniquefluorescine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene nameFc fragment of IgG binding protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameFc gamma receptor Ia
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Synonyms gene name
- Fc fragment of IgG, high affinity Ia, receptor for (CD64)
- Fc fragment of IgG, high affinity Ia, receptor (CD64)
- Fc fragment of IgG receptor Ia
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-12-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameFc receptor like B
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Synonyms gene
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Synonyms gene name
- Fc receptor-like and mucin-like 2
- Fc receptor-like B
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-05-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data