Biotinylated AGE-BSA

  • Catalog number
    7929-250
  • Price
    Please ask
  • Size
    250 µg
  • Description
    AGEs can bind a variety of receptors. Biotinylation of AGE-BSA allows for its purification by Streptavidin-Sepharose beads. Advanced glycation end products (AGEs) accumulate with age and at an accelerated rate in diabetes. AGEs bind cell-surface receptors including the receptor for AGE (RAGE). AGEs therefore have been implicated in the pathogenesis of diabetes, induction of proinflammatory cytokines, and stimulation of smooth muscle proliferation, and fibronectin production. The Biotinylated AGE-BSA was produced by biotinylation of AGE-BSA (Cat. # 2221-10) with Sulfo-NHS-LC-Biotin (Cat. # 2326). Prior to biotinylation, AGE-BSA was produced by reacting BSA with Glycoaldehyde under sterile conditions followed by extensive dialysis and purification steps. Fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex./Em. = 370/440 nm. Glycated BSA shows a 7000% increase in fluorescence in compared to control BSA.
  • Product Highlights
    FORM: Liquid. FORMULATION: 5 mg/ml of 0.22 µm filter sterilized solution in 1X PBS LABELING EFFICIENCY: BioVision’s Biotinylated AGE-BSA was estimated to contain ~1-2 biotin/BSA (As tested using Biotin Quantitation Kit (K811-100)). APPLICATIONS: Biotinylation of AGE-BSA allows for its pull-down and purification by Streptavidin-Sepharose beads (Cat. # 6565).
  • Storage Temp
    -70°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Notes
    For research use only. Not to be used for human or animal treatment or consumption.
  • Gene target
  • Short name
    Biotinylated AGE-BSA
  • Alternative name
    Biotinylated AGE-cow serum albumin
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