Sulfo-NHS-Biotin
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Catalog number2322-50
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PricePlease ask
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Size50 mg
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Alternative_nameBiotin 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfosuccinimidobiotin; Sulfo NHS Biotin;Sulfosuccinimidyl biotin; N-hydroxysulfosuccinimidyl biotin; N-hydroxysulfosuccinimido biotin
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DescriptionKEY FEATURES: • Sulfo-NHS-Biotin is amine-reactive and has a spacer arm length of 13.5 Å. • Water-soluble; can be used under physiologic conditions • The amide bond formed is permanent and the alkyl spacer arm cannot be cleaved APPLICATIONS: • Sulfo-NHS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
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CAS Number119616-38-5
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Structure AvailableYes
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Salt FormNo
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Molecular FormulaC₁₄H₁₈O₈N₃S₂Na
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Molecular Weight443.43
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Cell PermeableNo
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Purity≥85% by NMR
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SolubilitiesH₂O (~ 20 mg/ml)
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HandlingProtect from moisture
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Tag LineA water-soluble biotinylating reagent
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Storage Condition-20 ̊C
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Shipping ConditionGel Pack
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Shelf Life36 months
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MDL NumberMFCD00078535
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PubChem CID16219034
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SMILESC1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na]
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InChiInChI=1S/C14H19N3O8S2.Na/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);
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InChi KeyWEECOOCIRDDPES-UHFFFAOYSA-N
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MSDS AvailableYes
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Gene target
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Gene symbolNHS, NHS-AS1
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Short nameSulfo-NHS-
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LabelBiotin
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Alternative nameSulfo-NHS-biotinilated
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Gene info
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Identity
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Gene
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Long gene nameNHS actin remodeling regulator
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Synonyms gene name
- Nance-Horan syndrome (congenital cataracts and dental anomalies)
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNHS antisense RNA 1
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Synonyms gene name
- NHS antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-07-29
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Classification
- Antisense RNAs
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VEGA ID