Biotin LC NHS for cell activation

Biotin LC NHS for cell activation is available 3 times from Biovision labs

2345-50 | Biotin-LC-NHS for cell activationsize: 50 mg | 161.75 USD

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2345-1000 | Biotin-LC-NHS for cell activationsize: 1 g | 1141.38 USD

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2345-250 | Biotin-LC-NHS for cell activationsize: 250 mg | 478.42 USD

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  • Description
    Biotin-LC-NHS is a useful reagent for biotinylation of primary amines under alkaline conditions (pH~8-9). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc. Inserts a 6-atom spacer between biotin and the target ligand, thus minimizing the steric hindrance.
  • Peptide sequence
    N/A
  • CAS number
    72040-63-2
  • Molecular weight
    454.54
  • Category
    Biochemicals
  • Other name
    Biotin-6-aminohexanoic acid-N-hydroxysuccinimide ester; Biotin-X-NHS
  • Molecular formula
    C₂₀H₃₀N₄O₆S
  • Physical appearance
    White solid
  • Supplied with
    N/A
  • Is this a salt?
    No
  • Is it cell-permeable?
    No
  • Purification
    ≥95% by HPLC
  • Reconstitute instructions
    DMSO (20 mg/ml) or DMF (20 mg/ml)
  • Storage condition
    -20 ̊C
  • Shipping condition
    gel pack
  • Maximum time for storage
    36 months
  • Storage instructions
    Protect from air and moisture
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Additional description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
  • Short name
    -LC-NHS for activation
  • Technique
    activation
  • Label
    Biotin
  • Alternative name
    biotinilated-LC-NHS to measure cellular activation
  • Tissue
    cell
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