Biotin-LC-LC-NHS
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Catalog number2346-5000
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PricePlease ask
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Size5 g
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Alternative_name6-((6-((Biotinoyl)amino)hexanoyl)amino)hexanoic acid, succinimidyl ester; Biotin-XX-NHS
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DescriptionBiotin-LC-LC-NHS is a useful reagent for biotinylation of primary amines under mild conditions (pH~6.5-8.5). Some examples of primary amines for biotinylation include lysines on the surface of proteins, antibodies, etc. Inserts a 14-atom spacer between biotin and the target ligand, thus minimizing the steric hindrance in binding avidin or streptavidin to some biotinylated compounds and increase sensitivity for detection.
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CAS Number89889-52-1
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Structure AvailableYes
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Salt FormNo
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Molecular FormulaC₂₆H₄₁NO₇S
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Molecular Weight567.7
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Cell PermeableNo
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Purity≥95% by HPLC
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SolubilitiesDMF (~ 25 mg/ml)
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HandlingProtect from air and moisture
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Tag LineA biotinylation agent for primary amines
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Storage Condition-20 ̊C
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Shipping Conditiongel pack
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Shelf Life36 months
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MDL NumberMFCD00467154
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PubChem CID16219024
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SMILESC1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
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InChiInChI=1S/C26H41N5O7S/c32-20(27-16-8-2-4-12-24(36)38-31-22(34)13-14-23(31)35)10-3-1-7-15-28-21(33)11-6-5-9-19-25-18(17-39-19)29-26(37)30-25/h18-19,25H,1-17H2,(H,27,32)(H,28,33)(H2,29,30,37)/t18-,19-,25-/m0/s1
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InChi KeyATYCFNRXENKXSE-MHPIHPPYSA-N
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MSDS AvailableYes
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Gene target
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Gene symbolPTPN7, CT83, NHS, NHS-AS1
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Short name-LC-LC-NHS
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LabelBiotin
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Alternative namebiotinilated-LC-LC-NHS
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Gene info
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Identity
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Gene
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Long gene nameprotein tyrosine phosphatase non-receptor type 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-03-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein tyrosine phosphatases non-receptor type
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecancer/testis antigen 83
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Synonyms gene
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Synonyms gene name
- chromosome X open reading frame 61
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2007-06-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNHS actin remodeling regulator
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Synonyms gene name
- Nance-Horan syndrome (congenital cataracts and dental anomalies)
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameNHS antisense RNA 1
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Synonyms gene name
- NHS antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-07-29
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Classification
- Antisense RNAs
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VEGA ID