BioTeZ Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg antibody/ other protein Biotin Labelling Kit Kit

• Catalog number
  BTBIOK-05
• Price
  Please ask
• Size
  For 5 labels

• ELISA category
  Biotin Labelling Kit Kit
• ELISA detection limits
  N/A
• ELISA sensitivity
  N/A
• ELISA s description
  The BioTeZ Biotinylation Kit contains the reagents for the labeling of antibodies and other proteins and the purification of the biotinylated product. The coupling reaction takes place at primary amino groups on the target molecule using a N-Hydroxysuccinimide (NHS) ester-activated biotin, which form stable amide bonds. Proteins generally have a sufficient number of amino groups for coupling, both N-terminal and lysine residues. In this kit, Sulfo-NHS-LC Biotin (sulfosuccinimidyl-6-[biotin-amido] hexanoate) is used, that contains a spacer group with 6 carbon atoms and a length of 22,4 Å to increase the distance between the target molecule and the biotin groups. In the kit are 5 portions of Sulfo-NHS-LC Biotin reagent. One portion is used for a antibody amount of 1-10 mg or a protein amount of 1-5 mg.
• Research area interests
  all immuno assays
• Package form
  Kit
• Antigen
  Streptavidin
• Reacts with
  Streptavidin
• Tested applications
  Biotin Labelling , e.g. for antibodies, proteins etc.; Biotinylated molecules bind very well to Streptavidin or Streptavidin surfaces
• Specificity
  Streptavidin
• Crossreactivity
  N/A
• Preservative materials
  If applicable mentioned in the product data sheet
• Storage recommendations
  12 month
• Shipping and storage conditions
  > 0 to +37°C
• Long term storage
  2-8°C/ -20°C
• Assay principle
  Biotinylation with Sulfo-NHS-LC of antibody / protein, coupling reaction with target molecules through the reaction with primary amino groups
• Included in the standard package
  Sulfo-NHS-LC Biotin, biotinylation buffer for the coupling reaction, clean-up column and purification buffer concentrate (10x) for size-exclusion chromatography. All components are included for 5 separate coupling reactions.
• Not included in the standard package
  Pipettes or pipetting equipment, vials for dilution, distilled or deionised water
• Samples to be analyzed
  Biotinylation of antibody orprotein
• PubMed citations
  See the datasheet
• Technical datasheet
  Contact us to receive the datasheet
• Representative figures
  No Available
• Warnings
  For Research Use only.
• Other products
• Properties
  If you buy Antibodies supplied by biotez they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Description
  Antibody labelling kits.
• French translation
  anticorps

• Gene target
  BioTeZ   Biotinylation   Kit   Reagents   for   the   Biotinylation   other   protein   Kit   Kit  
• Short name
  BioTeZ Biotinylation Kit; Reagents for the Biotinylation of 1 – 10 mg antibody/ other protein Kit Kit
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Label
  Biotin
• Alternative name
  BioTeZ Biotinylation reagent; Reagents to measure the Biotinylation on 1 – 10 mg (antibody to-)/ other protein biotinilated Labelling reagent reagent
• Alternative technique
  antibodies, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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