Beta Actin Antibody Loading Control

  • Catalog number
    N1037-100UG
  • Price
    Please ask
  • Size
    0.1mg
  • Category
    Antibody
  • Concentration
    0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
  • Form
    Purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    Beta Actin Loading Control
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    Human (Homo sapiens), Mouse (Mus musculus), Rat, Monkey, Rabbit (Oryctolagus cuniculus), Chicken, Dog ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB
  • Recommended dilutions
    Western blot: 1-3ug/ml
  • Notes
    Provided assay concentrations are suggestions only, beta Actin antibody titration may be required for optimal results.
  • Added buffer
    1X Phosphate Buffered Saline (PBS), pH 7.4
  • Intented use
    This beta Actin antibody loading controlis to be used only for research purposes and not for diagnostics..
  • Gene ID
    60
  • Purity
    Purified by protein A Affinity Chromatography
  • Description
    Actins proteins are highly conserved and involved in cellular structure, integrity and motility. There are three main isoforms of actin in vertebrates: alpha, beta and gamma. Alpha actins are found in muscle cells while beta and gamma actins are non-muscle actins. Beta actin is commonly used as a loading control protein in western blot. An antibody to beta actin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
  • Immunogen
    A recombinant protein fragment from the N-terminus of human beta Actin was used as the immunogen for this antibody.
  • Storage
    Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Additional description
    Isotype or positive controls by peptides, antibodies and deactivated samples.
  • French translation
    anticorps
  • Gene target
    Beta   Actin   Loading  
  • Short name
    Anti-Beta Actin Loading Control
  • Technique
    Antibody, Control, antibodies against human proteins, antibodies for, controls
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibodies to Beta Actin Loading Control
  • Alternative technique
    antibodies, controls
  • Tissue
    control
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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