Glycine Oxidase H244K, Bacillus subtilis recombinant
-
Catalog number
7845-1000
-
Price
Please ask
-
Size
1 mg
-
-
Synonyms
Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
-
Alternative_names
Glycine oxidase, glycine oxygen oxidoreductase (deaminating), GO
-
Description
≥90% Pure Active Bacillus subtilis recombinant Glycine Oxidase H244K which shows a higher substrate specificity ratio for glycine versus sarcosine
-
Recombinant
Yes
-
Source
E. coli
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Activity Specifications test method
1200 mU/mg
-
Biological activity
1200 mU/mg
-
Results
1200 mU/mg
-
Unit Definition
One unit is defined as the amount of enzyme required to convert one micromole of glycine into glyoxylate and hydrogen peroxide at pH 8.5 at 37°C.
-
Molecular Weight
43.1 kDa (1-369 aa, NT His Tag)
-
Storage Temp
-20°C
-
Shipping
gel pack
-
Shelf Life
12 months
-
Concentration
4.0 mg/ml
-
Appearance
Liquid
-
Physical form description
4.0 mg/ml in 20 mM potassium phosphate, 100 mM NaCl, pH 8.0 and 10% glycerol
-
Background Information
Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.
-
Amino acid sequence
1-369 aa
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Additional description
Bacillus Anthracis and Cereus are the most known Bacilli. Anthrax has a very high toxin level and is used in Biologic warfare. However 1 is a harmless product.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Short name
Glycine Oxidase H244K, Bacillus subtilis recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative name
Glycine Oxidase H244K, Bacillus subtilis Rec.
-
Alternative technique
rec
-
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products