BAM, Beta-amylase, Biotin conjugated

• Catalog number
  AS09 380
• Price
  Please ask
• Size
  1 ml (10 mg/ml)

• Available ordering format
  Lyophilized in PBS labelled with biotin
• Immunogen
  Beta amylase isolated and purified from sweet potato Q94EU9
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Purified IgG
• How to reconstitute
  For reconstitution please add 1 ml of sterile destilled water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), ELISA (ELISA), immunofluorescence (IF), immunohistochemistry (IHC)
• Connected products
  AS09 379 | anti-beta amylase antibodyPlant and algal protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 1000 to 1: 4000 for techniques listed above
• Molecular weight expected аpparent
  60 kDa
• Verified reactivity
  Solanum tuberosum
• Possible reactivity
  dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis, Vitis vinifera, trees: Populus trichocarpa, Physcomitrella patens
• No reactivity
  n.a.
• Supplementary information
  antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
• References
  not available at the moment
• Scientific context
  Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
• Notes
  biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidobiotin.
• Protein number
  Q94EU9
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated

• Gene target
  BAM   Beta-amylase   conjugated  
• Gene symbol
  SMC3
• Short name
  BAM, Beta-amylase, conjugated
• Technique
  antibody Conjugates
• Label
  Biotin
• Alternative name
  BAM, b-amylase, biotinilated coupled

Gene info

• Identity
  HGNC:2468
• Gene
  SMC3
• Long gene name
  structural maintenance of chromosomes 3
• Synonyms gene
  • CSPG6
• Synonyms gene name
  • chondroitin sulfate proteoglycan 6 (bamacan)
• Synonyms
  • HCAP
  • BAM
  • SMC3L1
  • bamacan
• Synonyms name
  • bamacan proteoglycan
• GenBank acession
  • AF020043
• Locus
  10q25.2
• Discovery year
  1999-08-20
• Entrez gene record
  9126
• Pubmed identfication
  • 9506951
  • 10358101
  • 17273969
• RefSeq identity
  • NM_005445
• Classification
  • Proteoglycans
  • Structural maintenance of chromosomes proteins
  • Cohesin complex
• VEGA ID
  OTTHUMG00000019042
• Locus Specific Databases
  • Global Variome shared LOVD
  • LRG_774

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