B7DC antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Monoclonal Antibodies
Area of research

Immunology
Raised in

Rat
Specificity

Mouse
Isotypes

IgG2a kappa
Clone

TY25
Conjugate Tag

Biotin
Concentration

500 ug/ml
Form Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
Storage

Store at 2-8 deg C. Do not freeze. Protect from light.
Shipping conditions

Blue Ice
Tested for

FC
Usage Recommendations

FC: ≤ 0.5 ug/test
Assay Information

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data