B-Cell, Unclustered, 170kD, Clone: DF-B1, Mouse Monoclonal antibody-Human; flow, IF, IP
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Catalog numberMEDCLA265
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PricePlease ask
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Size1000ul
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Alternative name 1B-Cell, Unclustered, 170kD, Clone: DF-B1, Mab anti-Human; flow, IF, IP
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Alternative name 2B-Cell, Unclustered, 170kD, Clone: DF-B1, Mab antibody-Human; flow, IF, IP
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Alternative name 3B-Cell, Unclustered, 170kD, Clone: DF-B1, Mab antibody-Homo sapiens; flow, IF, IP
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Alternative name 4anti-B-Cell Unclustered 170kD Clone: DF-B1 flow IP
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Alternative name 5B-Cell Unclustered 170kD Clone: DF-B1 flow IP
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
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TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolBBS9, SNAR-B1, CFD, PCDHGB1, STON1, MS4A1, SH3GL2, VCX, NABP2
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Short nameB- , Unclustered, 170kD, Clone: DF-B1, Mouse Monoclonal antibody- ; , IF, IP
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TechniqueMouse monoclonal, Antibody, Mouse, Flow, IF, IP, antibodies against human proteins, antibodies for, Immunoprecipitation, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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SpeciesHuman, Humans, Mouses
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Alternative nameB-cellular, Unclustered, 170kD, clonality: DF-B1, Mouse monoclonal (antibody to-)-H. sapiens; flow, IF, IP
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Alternative techniqueantibodies, murine
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameBardet-Biedl syndrome 9
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Synonyms
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Synonyms name
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Locus
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Discovery year2007-01-18
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Entrez gene record
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Pubmed identfication
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Classification
- BBSome
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall NF90 (ILF3) associated RNA B1
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Synonyms gene name
- small ILF3/NF90-associated RNA B1
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GenBank acession
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Locus
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Discovery year2008-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small NF90 (ILF3) associated RNAs
Gene info
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Identity
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Gene
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Long gene namecomplement factor D
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Synonyms gene
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Synonyms gene name
- D component of complement (adipsin)
- properdin factor D
- complement factor D (adipsin)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-10-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Granule associated serine proteases of immune defence
- Complement system activation components
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprotocadherin gamma subfamily B, 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Clustered protocadherins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namestonin 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-02-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemembrane spanning 4-domains A1
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Synonyms gene
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Synonyms gene name
- membrane-spanning 4-domains, subfamily A, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-05-23
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Entrez gene record
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Pubmed identfication
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Classification
- Membrane spanning 4-domains
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameSH3 domain containing GRB2 like 2, endophilin A1
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Synonyms gene name
- SH3 domain containing GRB2 like 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- N-BAR domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namevariable charge X-linked
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Synonyms gene name
- variable charge, X chromosome
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-03-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Variable charge X/Y family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenucleic acid binding protein 2
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Synonyms gene
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Synonyms gene name
- oligonucleotide/oligosaccharide-binding fold containing 2B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-01-17
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data