Proteinase K, Recombinant, NGS Grade

  • Catalog number
    9247-100
  • Price
    Please ask
  • Size
    100 mg
  • Synonyms
    Endopeptidase K, Tritirachium alkaline proteinase
  • Alternative_names
    Protease K, Endopeptidase K, Tritirachium alkaline proteinase, NGS Grade Proteinase K, NGS Grade Protease K
  • Description
    A serine protease which inactivates nucleases
  • Recombinant
    Yes
  • Source
    Tritirachium album
  • Purity by SDS PAGE
    Highly purified
  • Activity Specifications test method
    ≥ 35 U/mg lyophilizate; ≥ 45 U/mg protein; ≥ 900 U/ml liquid
  • Unit Definition
    One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 umol tyrosine per 1 min at 37°C and pH = 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.
  • Molecular Weight
    28.9 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    24 months
  • Appearance
    Lyophilized Powder
  • Physical form description
    White lyophilized powder
  • Reconstitution Instructions
    In water
  • Background Information
    A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene
    Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    Proteinase   NGS   Grade  
  • Short name
    Proteinase K, Recombinant, NGS Grade
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Alternative name
    Proteinase K, Rec., NGS Grade
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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